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| ONLINE |
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1347-5223 |
| PRINT |
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0009-2363 | | |
| CHEMICAL & PHARMACEUTICAL BULLETIN |
| Vol. 55 (2007) , No. 9 1291 | |
[PDF (642K)] [References]  |
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Pharmacokinetics of Isofraxidin in Rat Plasma after Oral Administration of the Extract of Acanthopanax senticosus Using HPLC with Solid Phase Extraction Method
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| Hui Sun1)2), Haitao Lv1), Yanmei Zhang1), Xijun Wang1), Kaishun Bi2) and Hongxin Cao3) | |
1) College of Pharmacy, Heilongjiang University of Chinese Medicine
2) School of Pharmacy, Shenyang Pharmaceutical University
3) China Academy of Chinese Medical Science | |
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(Received February 2, 2007)
(Accepted May 31, 2007)
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High-performance liquid chromatography coupled with solid phase extraction method was developed for determination of isofraxidin in rat plasma after oral administration of Acanthopanax senticosus extract (ASE), and pharmacokinetic parameters of isofraxidin either in ASE or pure compound were measured. The HPLC analysis was performed on a Dikma Diamonsil RP18 column (4.6 mm×150 mm, 5 μm) with the isocratic elution of solvent A (acetonitrile) and solvent B (0.1% aqueous phosphoric acid, v/v) (A : B = 22 : 78) and the detection wavelength was set at 343 nm. The calibration curve was linear over the range of 0.156—15.625 μg/ml. The limit of detection was 60 ng/ml. The intra-day precision was 5.8%, and the inter-day precision was 6.0%. The recovery was 87.30±1.73%. When the dosage of ASE is equal to pure compound caculated by the amount of isofraxidin, it has been found to have two maximum concentrations in plasma while the pure compound only showed one peak in the plasma concentration–time curve. The determined content of isofraxidin in plasma after oral administration of ASE is the total contents of free isofraxidin and its precursors in ASE in vitro. The pharmacokinetic characteristics of ASE showed the priority of the extract and the properities of traditional Chinese medicine. | |
| Key words |
pharmacokinetics; isofraxidin; Acanthopanax senticosus; high-performance liquid chromatography; solid phase extraction | | |
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To cite this article: |
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Hui Sun, Haitao Lv, Yanmei Zhang, Xijun Wang, Kaishun Bi and Hongxin Cao, “Pharmacokinetics of Isofraxidin in Rat Plasma after Oral Administration of the Extract of Acanthopanax senticosus Using HPLC with Solid Phase Extraction Method”, Chem. Pharm. Bull., Vol. 55, 1291-1295 (2007) . |
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doi:10.1248/cpb.55.1291 |
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JOI JST.JSTAGE/cpb/55.1291 |
| (c) 2007 The Pharmaceutical Society of Japan | |
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